S. Lodato, Humanitas University - Clinical and Research Center, IRCCS - Milan

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Data dell'evento: 22/03/2018
seminars_2018

Friday, 23th March – h. 14:30
Seminars Room, NICO

Simona Lodato, PhD
Dep. of Biomedical Sciences, Humanitas University- Humanitas Clinical and Research Center, IRCCS – Milan

Decoding neuronal diversity of the mammalian cerebral cortex in development, evolution and disease

The mammalian cerebral cortex contains an unparalleled diversity of neuronal subtypes, which is the ultimate result of complex, spatially and temporally controlled differentiation processes of defined pool of neuronal progenitors. While the distinct molecular logics and the defining principles that control the generation of such heterogeneous neuronal output have just begun to be elucidated, it is well appreciated that multiple genetic and environmental factors concur to the establishment of distinct classes of both excitatory projection neurons (PNs) and inhibitory interneurons (INs) that populate the cerebral cortex. Balanced activity indeed relies on the precise assembly of specialized neuronal circuits that involve PN and IN subtypes.
 
Although they have different developmental origins, these two classes of neurons ultimately co-reside in the cortex,  where they assemble into functional local microcircuitry. The developmental events governing the proper interaction between PNs and INs are poorly understood, in particular the cellular and molecular events that direct interneurons to position precisely within specific cortical layers. We have previously reported that the acquisition of a distinct subtype-specific PN identity uniquely and differentially determines the development of cortical microcircuit assembly.

We are currently investigating the intrinsic molecular mechanisms and activity-dependent mechanisms that control specific pairing between distinct subtypes of PNs and INs in the early stages of radial migration of cortical interneurons. To address this question, we FACS-purified and transcriptionally profiled PN and IN subtypes, over multiple developmental time points from both murine (embryonic and postnatal) and human corteces (fetal). In particular, by combining standard isolation strategies to a recently developed purification method that does not require genetic labelling of the distinct neuronal subtypes, we are building a critical resource of gene expression data from multiple subtypes of PNs and INs isolated from the murine neocortex and, in parallel, from human fetal cortex.

Using custom bioinformatic analysis we aim at identifying evolutionally conserved (as well as species specific) principles of diversity generation, while identifying candidates potentially relevant for the lamination and integration of  interneurons within the cortex. this will shed light on the molecular logics behind assembly of specialized cortical circuits, which are often targets of devastating neurodevelopmental disorders

Host: Alessandro Vercelli

Agenda

21 gennaio 2022
06 aprile 2022

94° Congresso SIBS - Società Italiana di Biologia Sperimentale Torino, 6 - 9 aprile 2022

Ambiente e salute, oncologia, invecchiamento sano e attivo. Ma anche Antropologia, Biologia degli ambienti acquatici e Intelligenza artificiale. Sono solo alcuni dei temi protagonisti del 94° Congresso SIBS - Società Italiana di Biologia Sperimentale - ospitato a Torino dal 6 al 9 aprile 2022 e organizzato da Marina Boido e Corrado Calì, NICO e Dipartimento di Neuroscienze dell'Università di Torino.

Sportello LESIONI SPINALI

Mandateci le vostre domande

Premesso che al NICO ci occupiamo di ricerca di base (e non abbiamo quindi pazienti in cura), crediamo sia compito di noi ricercatori dare risposte chiare e soprattutto scientificamente corrette a chi incorre in notizie parzialmente false, scorrette o addirittura in vere e proprie bufale.
Il caso Stamina ci ha insegnato a essere vigili: un piccolo soffio di vento, se “seminato” in un terreno fertile, può infatti scatenare una pericolosa tempesta.

Da qui la volontà di creare questo sportello, uno spazio dedicato alle vostre domande e ai vostri dubbi. Un progetto nato in sinergia con il Coordinamento Para-Tetraplegici del Piemonte, con cui siamo in contatto da molto tempo, e con il Prof. Diego Garbossa, Primario di Neurochirurgia di Città della Salute e della Scienza di Torino, con cui abbiamo creato un team che riunisce ricerca base e neurochirurgia.

Inviate le vostre domande e le leggete alcune delle nostre risposte

31 agosto 2020