J. Pasterkamp, University Medical Center Utrecht

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Data dell'evento: 21/03/2018
seminars_2018

Thursday, 22nd March – h. 14:30
Seminars Room, NICO

Jeroen Pasterkamp
Professor of Translational Neuroscience, University Medical Center Utrecht (The Netherlands)
Director MIND Facility | Chair Brain (Center Rudolf Magnus)

Molecular mechanisms of neural circuit development

The midbrain dopamine system is involved in the control of cognitive and motor behavior. Midbrain dopamine neurons (mDA) are grossly divided into two anatomically and functionally distinct subpopulations: substantia nigra pars compacta (SNc) and ventral tegmental area (VTA) neurons. SNc neurons make precise connections with dorsal striatum (nigrostriatal projections), while VTA neurons target ventral striatum and cortex (mesocorticolimbic projections). Both pathways collectively run in the medial forebrain bundle (MFB) towards the forebrain. However, how mDA neurons establish their complex connections remains poorly understood. Therefore, we use the mDA system to examine how axons find their way during development, how neurons migrate and how ultimately synaptic connections are established.

In this lecture, I’ll cover two topics. First, novel axon-dependent mechanisms for mDA axon pathfinding that rely on specific cell adhesion molecules, and 2) recently developed mouse genetics tools to distinguish between different subsets of dopaminergic projections in vivo (called Pitx3-ITC mice).

The subtractive genetic strategy we have developed relies on the expression of different fluorescent proteins in different subsets of mDA neurons in a single mouse. Pitx3-ITC mice display labeling of SNc neurons and selective visualization of nigrostriatal projections in the MFB and in striatum, from early embryonic development onwards. Combination of Pitx3-ITC mice with 3D-imaging of solvent cleared organs (3DISCO) technology and light sheet imaging allows for 3D analysis of neuronal migration and axonal/dendritic development of SNc neurons.

Host: Silvia De Marchis

Agenda

30 marzo 2020
10 aprile 2020

Technical Report NICO

Un appuntamento per far conoscere potenzialità e modalità d'uso degli strumenti acquisiti recentemente dal NICO e favorire le collaborazioni fra i gruppi. 

Sportello LESIONI SPINALI

Paziente tetraplegico operato al CTO di Torino torna a usare le mani

Una notizia che abbiamo letto tutti di recente sui giornali. Noi del NICO abbiamo la fortuna di collaborare da anni con il dott. Bruno Battiston e il prof. Diego Garbossa - i chirurghi che hanno svolto l'operazione - e ci siamo fatti spiegare bene in cosa consiste l’intervento, quale era la situazione del paziente e quali saranno i possibili risultati. 
Tutto questo al di là delle semplificazioni e dei titoli roboanti dei giornali, comprensibilmente utilizzati per attirare l’attenzione dei lettori.

10 giugno 2019