Gruppo guidato da Carola Eva
Alessandra Oberto
Contacts:
Tel +39 011 670 6608
Fax +39 011 236 7718
e-mail: alessandra.oberto@unito.it
Curriculum Vitae
Born in Alba on 24.10.1967. Italian.
Education
2001 Assistant professor in Pharmacology and Toxicology, Department of Anatomy Pharmacology, and Forensic Medicine, Medical School, University of Turin, Italy
1996 PhDin Pharmacologyand Toxicology, University of Turin, Italy
1991 Master’s degree in Biology (summa cum laude), Faculty of Natural and Biological Sciences,University of Turin, Italy
Current position
Assistant professor (RU) in Pharmacology (BIO/14) Department of Neuroscience Rita Levi Montalcini and Neuroscience Institute Cavalieri Ottolenghi (NICO), University of Turin, Italy
Previous positions
2001-2003 Guest researcher, Max-Planck–Institute, Heidelberg. Laboratory of Molecular Neurobiology of Dr Rolf Sprengel. Research projects: 1) generation of conditional Y1R knock out transgenic mice using the doxicyclin regulated tet off system. 2) generation of Y1RVenus/Y5RtTA transgenic mice.
1999 Postdoctoral Fellowship. "Marie Curie Research Training Grant", Biotechnology program. Laboratory of Molecular Biology (supervisor Dr William Wisden) MRC, Medical Research Council, Dep. Neurobiology, Cambridge. Research projects: 1) in situ hybridization analysis of the expression of Y1 receptor mRNA in mice after anxiolytic and anxiogenic drug treatments. 2) Molecular biology techniques for making knock out mice using homologous recombination in Embryonic Stem cells.
1998 two years Postdoctoral Fellowship for Medical Science research, Institute of Pharmacology, Medical School. Project title: “Regulation of the Y1R gene expression from the hypotalamicsaziety factors”. PI: Prof. Carola Eva.
1994-1996: Visiting Fellow at Nathan S. Kline Institute (NYU) Center for Neuropharmacology, from September '94 to March '96. Studying the mechanism of Pb2+ neurotoxicity and its relationship to apoptotic neuronal death, under the direct supervision of Dr. Alessandro Guidotti.
1993-1994: Visiting Fellow FGIN, Georgetown University, Medical School, from March '93 to September '94, analyzing the gene promoter of an 18 Kd protein associated with the Mitochondrial Benzodiazepine Receptor complex in Dr. Karl Kruger lab.
1992-november 1996: PhD student in Pharmacology and Toxicology, under the supervision of Dr. Carola Eva. Institute of Pharmacology, Medical School, University of Turin. Thesis title: “Functional characterization of the promoter of the Y1 receptor mouse gene for neuropeptyde Y”.
1991: Guest Researcher from August 19th to November 2nd in the Laboratory of Cellular and Molecular Biology, NIH Bethesda. Engaged in Dr. Sandra Eva research project involving the bacterial expression and purification of the product of the dbl oncogene.
Teaching activities:
November 2001-2018: "Toxicology", Biotechnology School, University of Turin, Italy
November 2004-date: "Pharmacological Biotechnology", Molecular Biotechnology Master degree, University of Turin, Italy
November 2006-date: "General Pharmacology", Fundamentals of general pathology and physiopathology class, Degree forNeuropsychomotricityTerapistsof the developmental ages, University of Turin.
November 2006-2010: "General Pharmacology", First Aid class, Degree in Neurophysiopathology techniques, University of Turin
November 2010–date “Pharmacology”, Clinical and therapeutic methodology class, Nursery degree.
Scientific interests
Neuropeptide Y (NPY), hypothalamus, eating behavior, anxiety, mood disorders, obesity
The hypothalamic circuit of eating behavior, leptin and NPY. Generation of Y1R conditional knock-out mice. And modification of the Y1 and Y5 receptor gene to create mouse models for the study of anxiety, stress and eating disorders.
Neuroregulation and neuroprotective effect of NPY via Y1R. Epigenetic mechanisms that regulate cell differentiation during embryonic development. Maternal care as a tool to study the epigenetic modification of the Y1R gene.
NPY signaling role of estrogen dependent energy metabolism in female mammals.
Membership of scientific societies
SINS, Italian Society forNeuroscience
SFN, American Society for Neuroscience
Member of research centers
NeuroscienceInstitute Cavalieri Ottolenghi (NICO, Orbassano, Turin)
NIT, Centro Interdipartimentale di Studi Avanzati in Neuroscienze
Publications:
News
Il recettore NPY-Y1 controlla l'apprendimento spaziale e l'espressione delle reti perineuronali
Lo studio del nostro gruppo di Neuropsicofarmacologia - pubblicato su Neuropharmacology - suggerisce per la prima volta la presenza di un collegamento funzionale tra trasmissione NPY-Y1R e reti perineurali, un link funzionale che può svolgere un ruolo chiave nel controllo dell'eccitabilità dell’ippocampo dorsale e delle funzioni cognitive da esso regolate.
Le differenze di sesso influenzano l'impatto della manipolazione genica sul metabolismo e sul comportamento
Cervello e ormoni gonadici interagiscono nel controllare le funzioni metaboliche e comportamentali in modo sesso-dipendente. Tuttavia, la maggior parte della ricerca neuroscientifica traslazionale relativa a modelli animali di disturbi endocrini e psichiatrici viene spesso condotta solo su soggetti maschi.
In questa review le nostre ricercatrici del gruppo di Neuropsicofarmacologia suggeriscono come una maggiore conoscenza di tali differenze sia utile per lo sviluppo di terapie più mirate per una varietà di disturbi legati al sesso, come la sindrome metabolica, l’osteoporosi e la dipendenza da etanolo.
Maschi e femmine: uno studio sui meccanismi che regolano le differenze sessuali dell’attività cerebrale
Le differenze di sesso influenzano il cervello, il comportamento, le funzioni metaboliche e contribuiscono alla vulnerabilità alle malattie. Tuttavia, i meccanismi alla base delle differenze sessuali dell'attività cerebrale sono ancora poco noti.
Le nostre ricercatrici del gruppo di Neuropsicofarmacologia hanno analizzato l'inattivazione condizionale del gene Npy1r nei topi, osservando che induce differenze legate al sesso delle funzioni metaboliche e comportamentali.